Isolation And Characterization Of Ficus Bengalensis Fruit Mucilage Biology Essay

Natural polymers are advantageous over man-made polymers as they are economical, non-toxic, environment friendly, devoid of side effects, more patient conformity, etc. Mucilages are polysaccharide composites formed from sugar and uronic acid units. Mucilage signifier slimy multitudes in H2O, are typically heterogenous in composing. The mush of Ficus bengalensis contains a high proportion of mucilage and it besides being used for different curative intents. However there are no studies on isolation and word picture of mucilage of Ficus bengalensis. Hence, the present survey is planned to isolated and characterized for its morphological features, designation by chemical trials, Solubility, runing scope, pH, Swelling index, Ash values, presence of foreign organic affair, trial for lead and arsenic, Loss on drying, Density, squeezability index and angle of repose etc. which will be standardising parametric quantity for future research work.

Keywords: Ficus bengalensis, mucilage, isolation and word picture.

Introduction

The usage of natural gums and mucilage as of import portion of preparation is with the development of pharmaceutics and different dose signifiers. As general exicipients for unwritten usage, eg. in tablets and capsules etc. the options are limited1. The chances of natural polymers are brighter but even here extended testing will be required. The man-made polymers have certain disadvantages such as high cost, toxicity, environmental pollution during synthesis, non-renewable beginnings, side effects, less patient conformity, etc2. While the advantages of natural works based stuffs include low cost, natural beginning, free from side effects, bioacceptable, renewable beginning, environmental-friendly processing, local handiness ( particularly in developing states ) , better patient tolerance every bit good as public credence, from comestible beginnings, etc3. Mucilages are polysaccharide composites formed from sugar and uronic acid units. mucilages form slimed multitudes in H2O, are typically heterogenous in composing. Upon hydrolysis, arabinose, galactose, glucose, mannose, xylose and assorted uronic acids are the most often ascertained constituents. Mucilages are obtained chiefly from fruits. Some are obtained from marine algae, and from selected microorganisms4. In present survey the fruits of Ficus bengalensis. were selected for the isolation of mucilage. The fruits of Ficus bengalensis. besides known as Aliva in Marathi and garden cress in English, contain a high proportion of mucilage and it besides being used for different curative purposes5. The works exudates ( Acacia, karaya, and Tragacanth ) have been the traditional gums for pharmaceutical intents and they still find important applications. These gums are labour intensive and carry premium monetary value and their usage will likely go on to decline6. However there are no studies on isolation and word picture of mucilage of Ficus bengalensis. Hence, the present survey is planned to insulate and qualify mucilage of Ficus bengalensis.

MATERIAL AND METHOD:

Materials

The fresh Ficus bengalensis fruits were collected from workss turning in local countries of Anantapur, India. The works was authenticated at the Botany Department of Sri Krishnadevaraya University, Anantapur, India. Ethanol ( 95 % ) , Acetone, Tri chloro acetic acid, Na hydrated oxide and diethyl ether, were procured from SD Fine chemicals ( Mumbai, India ) . All other chemicals used were of analytical reagent class and dual distilled H2O was used throughout the experiments.

Extraction of mucilage

The fresh Ficus bengalensis fruits were collected and washed with H2O. Incisions were made on the fruits, left over dark. The fruits were crushed and soaked in H2O for 5-6 hours, boiled for 30 proceedingss and left to stand for 1 hr to let complete release of the mucilage into the H2O. The mucilage was extracted utilizing a multi-layer muslin fabric bag to take the marc from the solution. Acetone ( three times the volume of filtrate ) was added to precipitate the mucilage. The mucilage was separated, dried in an oven at 40A°C, collected, land, passed through a # 80 screen and stored in desiccator at 30A°C & A ; 45 % comparative humidness boulder clay use5. The gathered mucilage was tested for flow belongingss which were shown in Table 1. All values were found to be satisfactory.

Physical word picture of Ficus bengalensis fruit mucilage

Fresh Ficus bengalensis fruits were procured from the local market, Anantapur, India and authenticated by the Botany section, Sri Krishnadevaraya University, Anantapur, India. The seeds do non incorporate any mucilage and were removed prior to extraction. The fruits were sliced, homogenized with five times its weight of H2O, centrifuged at 4000 revolutions per minute for 15 min and the mucilage was precipitated with three volumes of ethyl alcohol and washed with more ethanol followed by propanone. The mucilage so obtained was dried under vacuity ( less than 1 Torr at 25A°C for 12 H ) .

Purification of the Mucilage

The petroleum mucilage ( 1 % ) was homogenized ( Potter homogenizer ) with cold dilute trichloro acetic acid solution ( 5 % ) . The solution was centrifuged ( 3500 revolutions per minute for 20 min ) , neutralized with Na hydrated oxide by bead wise add-on and so dialyzed for 30 H against distilled H2O. The mucilage was precipitated with ethyl alcohol ( in the measures of three times the volumes ) and washed in turn with ethyl alcohol, propanone and diethyl quintessence.

Word picture of Mucilage:

The gathered mucilage was evaluated for physicochemical features viz. , morphological features, designation by chemical trials, Solubility, runing scope, pH, Swelling index, Ash values, presence of foreign organic affair, trial for lead and arsenic, Loss on drying, Density, squeezability index and angle of repose etc. ( Table 1 ) The rating was carried out as per processs describe in official books.

RESULT AND DISCUSSION

Table 1: Physicochemical word picture of Abelmoschus esculentus fruit mucilage

Properties

Abelmoschus esculentus

fruit mucilage

Physical belongingss

Appearance

xanthous pulverization

Smell

Characteristic

Solubility

Slowly soluble in H2O

green goodss hage syrupy solution

% output ( g /kg )

23 A±2.173

Ave. atom size

165.15A±10.265

Wt. loss on drying

4.20A±2.573

Swelling ratio

45A±3.841

pH

7.0A±0.56

Charring ( 0C )

220A±4.52

Density of liquid ( 0.5 % w/v )

0.997A±0.055

Microbial count ( cfu/g )

Bacteria:5 ; Fungi: 2

Chemical belongingss

Mounted in 96 % ethyl alcohol

Transparent angular multitudes

Mounted in Ru ruddy

Atoms stained ruddy

Mounted in Iodine solution

Atoms stained bluish

Trial for Carbohydrate ( Mollish trial )

+

Trial for Tannins ( Ferric chloride trial )

Trial for chloride ( Silver-nitrate trial )

Trial for Sulphate ( Barium chloride trial )

Trial for Uronic acid

+

Trial for foreign affair ( % )

NMT 0.1

Trial for heavy metal ( lead )

23 ppm

Trial for Arsenic

& lt ; 1 ppm

Flow belongingss

Angle of rest ( qA° )

27.96A±1.684

Loose Bulk denseness ( g/cm3 )

0.604A±0.018

Tapped majority denseness ( g/cm3 )

0.866A±0.021

Carr ‘s Index

0.302A±0.023

Hausner ‘s ratio

1.434A±0.047

Number of tests ( N ) =5